Journal: Global Change Biology
Article Title: Rapid Evolution in Action: Environmental Filtering Supports Coral Adaptation to a Hot, Acidic, and Deoxygenated Extreme Habitat
doi: 10.1111/gcb.70103
Figure Lengend Snippet: Symbiodiniaceae communities along the environmental gradient. (a) NMDS of Wisconsin double standardized and 4th square‐root transformed ITS2 sequence abundance data. Colonies are colored by sampling site, and arrows represent ITS2 sequences. (b) Predicted relative abundance of Symbiodiniaceae ITS2 type profiles for each colony.
Article Snippet: We used ITS2 forward (5’‐TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGTGAATTGCAGAACTCCGTG‐3′) and ITS2 Reverse (5′‐GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCCTCCGCTTACTTATATGCTT‐3) Illumina‐tagged primers from Hume et al. (Hume et al. ) to amplify the ITS2 gene using DreamGreen PCR master mix (ThermoFisher Cat no. K1081) for a total of 35 PCR cycles.
Techniques: Transformation Assay, Sequencing, Sampling